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A standard calculation methodology for human doubly labeled water studies
Journal
Cell Reports Medicine
ISSN
2666-3791
Date Issued
2021-02-16
Author(s)
John R. Speakman
Yosuke Yamada
Hiroyuki Sagayama
Elena S.F. Berman
Philip N. Ainslie
Lene F. Andersen
Liam J. Anderson
Lenore Arab
Issaad Baddou
Kweku Bedu-Addo
Ellen E. Blaak
Stephane Blanc
Alberto G. Bonomi
Carlijn V.C. Bouten
Pascal Bovet
Maciej S. Buchowski
Nancy F. Butte
Stefan G.J.A. Camps
Graeme L. Close
Jamie A. Cooper
Seth A. Creasy
Sai Krupa Das
Richard Cooper
Lara R. Dugas
Cara B. Ebbeling
Ulf Ekelund
Sonja Entringer
Terrence Forrester
Barry W. Fudge
Annelies H. Goris
Michael Gurven
Catherine Hambly
Asmaa El Hamdouchi
Marije B. Hoos
Sumei Hu
Noorjehan Joonas
Annemiek M. Joosen
Peter Katzmarzyk
Kitty P. Kempen
Misaka Kimura
William E. Kraus
Robert F. Kushner
Estelle V. Lambert
William R. Leonard
Nader Lessan
David S. Ludwig
Corby K. Martin
Anine C. Medin
Erwin P. Meijer
James C. Morehen
James P. Morton
Marian L. Neuhouser
Theresa A. Nicklas
Robert M. Ojiambo
Kirsi H. Pietiläinen
Yannis P. Pitsiladis
Jacob Plange-Rhule
Guy Plasqui
Ross L. Prentice
Roberto A. Rabinovich
Susan B. Racette
David A. Raichlen
Eric Ravussin
Rebecca M. Reynolds
Susan B. Roberts
Albertine J. Schuit
Anders M. Sjödin
Eric Stice
Samuel S. Urlacher
Giulio Valenti
Ludo M. Van Etten
Edgar A. Van Mil
Jonathan C.K. Wells
George Wilson
Brian M. Wood
Jack Yanovski
Tsukasa Yoshida
Xueying Zhang
Alexia J. Murphy-Alford
Cornelia U. Loechl
Edward L. Melanson
Amy H. Luke
Herman Pontzer
Jennifer Rood
Dale A. Schoeller
Klaas R. Westerterp
William W. Wong
DOI
10.1016/j.xcrm.2021.100203
Abstract
The doubly labeled water (DLW) method measures total energy expenditure (TEE) in free-living subjects. Several equations are used to convert isotopic data into TEE. Using the International Atomic Energy Agency (IAEA) DLW database (5,756 measurements of adults and children), we show considerable variability is introduced by different equations. The estimated rCO2 is sensitive to the dilution space ratio (DSR) of the two isotopes. Based on performance in validation studies, we propose a new equation based on a new estimate of the mean DSR. The DSR is lower at low body masses (<10 kg). Using data for 1,021 babies and infants, we show that the DSR varies non-linearly with body mass between 0 and 10 kg. Using this relationship to predict DSR from weight provides an equation for rCO2 over this size range that agrees well with indirect calorimetry (average difference 0.64%; SD = 12.2%). We propose adoption of these equations in future studies.
Keywords: doubly labeled water; free-living; total energy expenditure; validation.
Keywords: doubly labeled water; free-living; total energy expenditure; validation.
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